| Microorganism name |
Trichoderma reesei
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| Target proteins |
Ovalbumin (Aro et al., 2023)
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| Wild-type or GMO |
GMO (Aro et al., 2023)
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| Production mode (intracellular/extracellular) |
Extracellular (Aro et al., 2023)
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| Protein yield (g/L or g/g?) |
2 g/L (Aro et al., 2023)
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| Temperature used in study |
28°C (Aro et al., 2023)
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| pH used in study |
pH 4.8-5.0 (Aro et al., 2023)
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| C & N source |
Glucose, yeast extract, (NH4)2SO4 (Aro et al., 2023)
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
Has been filed forFDA GRAS approval in the US. Not allowed in Canada
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| Companies |
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| Publications/references |
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Aro, N., Ercili-Cura, D., Andberg, M., Silventoinen, P., Lille, M., Hosia, W., Nordlund, E., & Landowski, C. P. (2023). Production of bovine beta-lactoglobulin and hen egg ovalbumin by Trichoderma reesei using precision fermentation technology and testing of their techno-functional properties. Food Research International, 163, 112131. https://doi.org/10.1016/j.foodres.2022.112132
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Nicolas Hardy, Frédéric Augier, Alvin W. Nienow, Catherine Béal, Fadhel Ben Chaabane. Scaleup agitation criteria for Trichoderma reesei fermentation. Chemical Engineering Science, 2017, 172, pp.158-168. ff10.1016/j.ces.2017.06.034ff. ffhal-01544606f
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Thiviya, P., Gamage, A., Kapilan, R., Merah, O., & Madhujith, T. (2022). Single Cell Protein Production Using Different Fruit Waste: A Review. Separations, 9(7), 178. https://doi.org/10.3390/separations9070178
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Alcantara, J., & Mondala, A. (2021). Optimization of Slurry Fermentation for Succinic Acid Production by Fungal Co-culture. DOAJ (DOAJ: Directory of Open Access Journals). https://doi.org/10.3303/cet2186255
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He, J., Wu, A., Chen, D., Yu, B., Mao, X., Zheng, P., Yu, J., & Tian, G. (2014). Cost-effective lignocellulolytic enzyme production by Trichoderma reesei on a cane molasses medium. Biotechnology for Biofuels, 7(1), 43. https://doi.org/10.1186/1754-6834-7-43
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Garvey, M. (2022). Non-Mammalian Eukaryotic Expression Systems Yeast and Fungi in the Production of Biologics. Journal of Fungi, 8(11), 1179. https://doi.org/10.3390/jof8111179
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Nakari-Setala, T., Penttila, M., Bailey, M., Tenkanen, M., & Individual. (2001, January). CA2382468A1 - A method for decreasing foam formation during cultivation of trichoderma - Google Patents. Retrieved August 22, 2025, from https://patents.google.com/patent/CA2382468A1/en
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Chai, S., Zhu, Z., Tian, E., Xiao, M., Wang, Y., Zou, G., & Zhou, Z. (2022). Building a Versatile Protein Production Platform Using Engineered Trichoderma reesei. ACS Synthetic Biology, 11(1), 486–496. https://doi.org/10.1021/acssynbio.1c00570
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